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This is because each additional week on semaglutide gives your GLP-1 receptors more time to adapt, and adaptation is cumulative even at lower doses

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Cells were pelleted at 300 g for 6 min, resuspended in complete MEM with 50 µg ml −1 Gen at 2.5 × 10 5 cells per ml, and dispensed (80 µl per well) into 384-well white and black plates (ThermoFisher) for luminescence and fluorescence measurements, respectively