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For detection of the chromatin-bounded PARP-1, the cells in 6 cm dishes were firstly lysed in 200 μL NETN 300 (20 mM Tris-HCl pH8.0, 300 mM NaCl, 1 mM EDTA, 0.5% NP40) buffer supplemented with protease inhibitor for 30 min on ice

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The image biomarker standardization initiative: standardized quantitative radiomics for high-throughput image-based phenotyping