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However, since cell counting is only a rough proxy to assess cytotoxicity and a higher dose escalation is necessary for CC 50 determination, we further included careful assessment of growth kinetics by live cell imaging, measurement of mitochondrial metabolic activity by MTT assay as well as cellular ATP levels by CellTiter-Glo (CTG, see “Material and Methods” section for details)

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Technical Skill Assessments in Cardiothoracic Surgery: Current State and Unfulfilled Need, 2020