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Before being embedded in paraffin wax, the muscle was fixed in buffered formalin (pH 7.4) for 24 h, decalcified with TBD-2 Decalcifier (ref

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The latter fact is important as it confirms the potential of IGF-1 DES to improve anabolism even in the setting of poor calorie intake, a function that would make the peptide useful in chronic disease and in individuals who are unable to consume a great deal of calories for a variety of reasons