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When adding water to the vial, direct the stream against the glass wall rather than directly onto the peptide cake, and swirl gently rather than shaking

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Catalase (CAT, EC 1.11.1.6, as H 2 O 2 -degrading enzyme), H 2 O 2 inhibitor diphenylene iodonium (DPI), and the NO inhibitor N w -nitro-L-arginine methyl ester (LNAME) partially inhibit UV-B-induced NO accumulation, indicating that H 2 O 2 and NOS-like activity play an important role in plant response to UV-B (Tossi et al., 2009)