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The cell lysates were then subjected to trichloroacetic acid precipitation followed by urea denaturation, reduction, blocking of disulfide bonds, and tryptic digestion (trypsin/protein ratio of 1:50 [wt/wt]) as described previously (81)

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[DOI] [PubMed] [Google Scholar] 88.Huang L, Nazarova EV, Tan S, Liu Y, Russell DG