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After incubation, the TMA coverslips were washed with the staining buffer for 2 min (2x) then the coverslips were incubated with post staining fixing solution (2% PFA) for 10 min at RT

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H '(20) = 49.5/20 = 2.475 cm/kg, while at a weight of 49.5 kg H '(49.5) = 49.5/49.5 = 1 cm/kg