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A microinjector (CellTram ® 4rOil, Eppendorf, Hamburg, Germany) equipped with a custom-made glass capillary was used to extract individual nephrocytes (for numbers of isolated cells, see Table 1) from the collagenase-treated ECM meshwork and directly transfer them into Roti ® Zol (Roth, Karlsruhe, Germany) for transcriptome analysis or lysis buffer for proteome analysis (iST sample preparation kit, Preomics, Planegg, Martinsried, Germany)

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Dovgan J