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Experimental design Animals were divided into 6 equal groups of five rats each as follows: group 1 served as the vehicle control and received distilled water by oral gavage (same volume as treated groups) once daily for 45 days, and groups from 2 to 6 were administered polystyrene microplastics at doses of 0.1, 1, 10, 20, and 40 µg/kg B.W