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The culture device was placed in a custom-made incubator that maintains a temperature of 37 °C, and tissue was maintained half-soaked in sterile, serum-free culture medium (Iscove’s Modified Dulbecco’s Medium without phenol-red (IMDM, GIBCO) supplemented with 20% KnockOut serum replacement (GIBCO), 2% B-27 and 1% of N-2 supplements (GIBCO), 1% L-glutamine, 1% non-essential amino acids, 1% HEPES) using a syringe pump