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Glutamine deprivation or inhibition of glutamine synthase in Caco-2 cells in culture significantly decreased the transepithelial electrical resistance (TER) and increased the paracellular permeability of the cell monolayer (52) and increased bacterial translocation (66)

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Ethical clinics therefore: Avoid exaggerated promises Present NAD+ as biologically plausible but variable in response Focus on individualised outcomes rather than guaranteed results This evidence-aware positioning distinguishes medical-grade NAD IV therapy in India from marketing-driven wellness content