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Macroautophagy and aging: the impact of cellular recycling on health and longevity

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In brief, the cells were plated at a density of 1.5 × 10 5 cells/cm 2 on 35 mm glass bottom petri dish, and after 7 days of culture, total ROS was quantified by staining the cultures with 10 uM CM-H 2 DCFDA working solution in HBSS