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The mechanism of JNK activation by morphine-like opioids was not fully defined, but agonist increases in phospho-JNK were blocked by the selective PKC inhibitor Gö6976 [12-(2-cyanoethyl)-6,7,12,13-tetrahydro-13-methyl-5-oxo-5 H -indolo(2,3- a )pyrrolo(3,4- c )carbazole], suggesting that PKC activation was required (Melief et al., 2010)