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Prior to LC-MS, the samples were transferred to column solvent and analyzed using a Thermo Q Exactive HF hybrid quadrupole/orbitrap high resolution mass spectrometer instrument on a Waters Symmetry 5 μ C18 column (150 × 2.1 mm) with an isocratic solvent of CH 3 CN/H 2 O/glacial acetic acid (50:50:0.01 by volume) at a flow rate of 0.3 ml/min