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a combination of 1 mM IBMX, 1 ⁇ M dexamethasone, and 10 ⁇ g/ml of insulin in media were added to fully confluent 3T3-L1 fibroblasts for three days (days 0-3) to initiate differentiation

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Vitamin B12 , meanwhile, may enhance mitochondrial energy production , translating to improved mood, focus, and motivation in patients who are fatigued or struggling with weight loss plateaus