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Therefore, the level of Fe 2+ in cells was detected by the iron content detection kit, and the mRNA expressions of ferritin light chain (FLC), ferritin heavy chain (FHC) and NCOA4 in cells were detected by qRT-PCR

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AIEC infection of intestinal epithelial cells induces ferroptosis through characteristic chemical features: reducing GPX4 expression while increasing lipid peroxide (LPO) accumulation [85]