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Normothermic perfusion: a mini-review

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Discussion Taken together, these results demonstrate that mimicking the hypoxic microenvironment of the developing brain during differentiation of human iPSCs into BMVECs, by either lowering oxygen levels or adding HIF1α-inducing mimetics (e.g., CoCl 2 or DMOG), enables differentiation of endothelial cells that recapitulate human-relevant physiological BBB properties when the cells are interfaced with human brain astrocytes and pericytes in a 2-channel microfluidic BBB Chip