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Cannulated Middle Cerebral Artery (MCA) Preparation: Rats were euthanized by pentobarbital overdose (Beuthanasia-D, Merck Animal Health, Summit, NJ) and middle cerebral arteries (MCA) were isolated and cleaned of connective tissue in physiological salt solution (PSS) containing 119 mM NaCl, 24 mM NaHCO 3 , 5.5 mM dextrose, 4.7 mM KCl, 1.6 mM CaCl 2 •2H 2 O, and 1.17 mM MgSO 4 •7H 2 O in ddH 2 O and adjusted to pH 7.4

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Ketogenic Diet Enhances Neurovascular Function with Altered Gut Microbiome in Young Healthy Mice