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The membrane was washed 3x for 15min in PBS-T and was then incubated with the secondary antibody for 1.5h at 25°C

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10.1186/s43094-020-00161-8 ↑ Singh A, Khan SA, Choudhary R, Bodakhe SH: Cinnamaldehyde Attenuates Cataractogenesis via Restoration of Hypertension and Oxidative Stress in Fructose-Fed Hypertensive rats