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After washing twice with UA as above, 100 μL of 50 mM AmBic (50 mM NH 4 HCO 3 in water) was added to the filter unit and the filter was centrifuged at 14,000× g for 10 min

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In mice given benzo [a] pyrene in the diet, malformations were found after administration of 120 mg/kg bw per day on days 2-10 of gestation