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This study applied injuries with AF puncture ∼80% IVD height through ∼50% IVD width to induce NP herniation, and this large and “criticallysized” injury was selected to cause IVD injuries shown to cause progressive IVD degeneration in adult mice and with detectable IVD biomechanical changes that are easier to detect than smaller injuries that do not consistently induce detectable biomechanical changes

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With social lives, work and screen time, sleep has turned into more of a nuisance than a necessity