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After, neurons were incubated in blocking solution containing anti-rabbit STAR635P (1:170 dilution, in-house conjugated rabbit Jackson ImmunoResearch 711-005-152 and STAR635P NHS ester Abberior 07679) and anti-mouse STAR580 (1:200 dilution, in-house conjugated mouse Jackson ImmunoResearch 715-005-151 and STAR580 NHS ester Abberior 38377) for 1 hour

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The chemical connection at the aspartic-acid residue also matters

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