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Isolation of WAT stromal vascular cells and adipocyte differentiation To isolate the stromal vascular fraction, minced iWAT (combined depots from 4–5 mice per genotype) from 4–6-week-old mice was digested in 1 mg ml −1 collagenase D (Roche) with 1.5% BSA in sterile Hank’s Balanced Salt Solution and incubated in a 37 °C shaking water bath for 1 h