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Endotoxin contamination — a common source of confounding in peptide research — can trigger inflammatory responses in biological assays that mimic or mask the compound’s actual biological activity

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Mass spectrometric analysis and detection were performed using specific MRM for analytes and their related labeled internal standards, on a Xevo TQ-XS (Waters ® , United States), equipped with electrospray ionization source in positive mode and argon as collision gas