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Briefly, the DAPI staining was used to create a nuclear mask, and fluorescence intensities of the stained markers (FOXO4, CDKN2A, CDKN1A, and TP53) and FOXO4 foci were measured within the nuclear mask

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Butterfield,"Proteomic Analysis of 4-Hydroxy-2-Nonenal-Modified Proteins in G93A-SOD1 Transgenic Mice - A Model of Familial Amytrophic Lateral Sclerosis," Free Radical Biology & Medicine 38, 960-968 (2005)