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To promote chromosomal integration, electroporation of the plasmid into Lm850658, and transformants were grown in BHI broth containing 10 μg/ml chloramphenicol at a non-permissive temperature of 42°C

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DNA methylation begins when the amino acid methionine is converted to that universal methyl donor, SAM, and concludes after enzymes called DNA methyltransferases (DNMTs) attach methyl groups from SAM to the base, cytosine