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Then, samples were first fixed with 50% of culture medium and 50% of 2% PFA with 2% of glutaraldehyde Hepes buffer 0.1 M at pH 7.4 for 1 h before being fixed with 2% of paraformaldehyde (PFA) with 2% of glutaraldehyde Hepes buffer 0.1 M for one night at 4 °C

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