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Additionally, alkaline phosphatase (ALP) and alizarin red S (ARS) staining was performed on cells cultured in osteogenic medium for at least 14 days

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In HCC cells, post-translational activation of CBS under TNFα-induced oxidative stress enhances TSS activity, mediated by a proteolytically cleaved and highly active form of CBS, enhancing cystathionine and GSH production [81]