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Rather than acting on a single pathway, slowing the nicotinamide-to-1-MNA conversion is theorized to leave more nicotinamide available for NAD+ synthesis while also protecting the cell’s broader methylation capacity, which affects gene expression and epigenetic regulation more broadly

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The two compounds operate on different timescales — BPC-157 in research protocols is frequently dosed daily, while TB-500 in many research traditions uses a loading phase followed by less frequent maintenance dosing