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The membranes were blocked 5% skim milk and probed with primary antibodies against anti-GSH antibody (ViroGen, Watertown, MA, USA, #101-A-250, 1:500) under non-reducing condition, Grx1 (Abcam, ab45953, 1:1,000), GR (ABclonal, Woburn, MA, USA, A2164, 1:1,000), GST-π (MBL, Schaumburg, IL, USA, 312, 1:1,000), Bax (Santa Cruz Biotechnology, CA, USA, sc-7480, 1:1000), Bcl2 (Santa Cruz Biotechnology, sc-7382, 1:1000), cleaved caspase-3 (Cell Signaling, Danvers, MA, USA, #9661, 1:1000), and anti-β-actin antibody (Santa Cruz Biotechnology, sc-47778, 1:1000;)

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Variations of the CxxC active site are associated with Trx superfamilies