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Using similar eluants, analytical UPLC-MS analyses were performed on System Ultimate 3000 UPLC (Thermofisher) equipped with a column Acquity peptide BEH300 C18 (1.7 µm, 2.1 × 100 mm), a diode array detector, a charged aerosol detector (CAD), and a mass spectrometer (Ion trap LCQfleet)

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Genome-scale measurement of off-target activity using Cas9 toxicity in high-throughput screens