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After intravenous administration of fluorescein, enhancement of the gaps between epithelial cells (1), leakage of fluorescein into the bowel lumen (2), and cell shedding (3) can be directly visualized using a confocal probe equipped with a 488 nm laser ( ex vivo permeability ( Finally, mucosal impedance testing is another tool to directly evaluate mucosal integrity, although most studies are limited to the esophagus