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The freshly treated hTrx1 has free thiol on surface Cys73 ( 2.7 Effect of thiol-reactive reagents on diamide-induced formation of TrxR1-hTrx1 complex The non-reduced TrxR1 was, respectively, pre-incubated with 10 mM H 2 O 2 , diamide, GSSG, GSNO, DTNB, IAM or IAA for 30 min at room temperature

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A: Benzyl alcohol is a bacteriostatic agent, meaning it slows or prevents bacterial growth without destroying microorganisms