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CICT The nuclear donor cells were prepared from the ear tissue of the CH2 homozygous genome-edited cat (Alsik) and were then immersed in cell culture medium (Dulbecco's modified Eagle's medium, DMEM, 11965, Gibco) supplemented with 15% FBS, 1% L-glutamine, 1% non-essential amino acids (11140–050, Gibco), 1% penicillin/streptomycin 15

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The canonical Wnt/β-catenin pathway is activated when Wnt ligands bind to Frizzled receptors and LRP5/6 co-receptors, resulting in the inactivation of the β-catenin destruction complex, which includes axin, APC, GSK3β, and CK1α