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If the research question requires comparing direct IGF-1R activation to GH-mediated IGF-1 synthesis, use IGF-1 LR3 in one arm and Ipamorelin in the other with identical dosing schedules and measurement time points

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Supernatants obtained from the lysates by centrifugation at 15000 x g/20 min/4°C were further diluted before using in colorimetry