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Reconstitution Protocol: Solvent: Sterile bacteriostatic water or appropriate buffer Technique: Add solvent slowly along vial wall Mixing: Gently swirl until dissolved—do not shake or vortex (shear forces damage peptide bonds) Sterility: Maintain aseptic conditions throughout Post-Reconstitution Storage: 2-8°C: Use within 4 weeks Aliquot and freeze at -20°C for extended storage Avoid repeated freeze-thaw cycles Protect from light and moisture The 3-Tier Storage Protocol STABLE - Prewritten Phase (Up to 4 Months) Unreconstituted lyophilized peptides remain chemically stable at room temperature (15-25°C) for 3-4 months when stored away from direct sunlight and moisture

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Nature 468, 696–700 (2010)