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Further, the SOE PCR products were cloned into FastDigest BshTI/FastDigest BglII (Thermo Fisher) linearized pEGFP-C2 vector using ClonExpress ® II One Step Cloning Kit (C112, Vazyme) to obtain the six sorting vectors, pEGFP-GST-GPI DAF , pEGFP-GST-GPI BY55 , pEGFP-GST-GPI CEAM7 , pEGFP-GST-TMD ITB3 , pEGFP-GST-TMD ITA5, and pEGFP-GST-TMD ITAV

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This is also why, by the way, it becomes more difficult to lose weight as you get older