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OCT frozen livers were cut in 8 µm-thick sections and fixed with 10% (v/v) non-buffered formalin for 2 min

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They were snap frozen in dry ice and lysed with 100 μl of JS Buffer (0.25 M HEPES pH 7, 0.15 M NaCl, 1% Glycerol, 1% Triton X-100, 0.0015 M MgCl2, 0.005 M EGTA) supplemented with EDTA-free protease inhibitors (Sigma Aldrich, #11873580001)