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As an additional line of evidence to substantiate these data, we utilised proximity ligation assays (PLA), a form of co-localisation in which punctate fluorescent signals are only generated if the two targeted antigens reside within a maximum of 40 nm of each other 72, 73
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Usage Period:365 days
Using pathway enrichment analysis, we found significant differences between the metabolites in tumors from vehicle- and JHU083-treated mice, such as in glycine and serine metabolism, phosphatidylcholine biosynthesis, methionine metabolism, urea cycle, glutamate metabolism, ammonia recycling, amino sugar metabolism, and arginine and proline metabolism pathways (Supplemental Figure 7C)