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Nitrated lipids, such as nitroalkenes, may undergo aqueous decay and release independent of thiols, isomerize to a nitrite ester with N-O bond cleavage, or generate an enol group and NO [Leopold & Loscalzo, 2009]

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When a ligand (an agonist in this case) binds to its GPCR, the GPCR undergoes a conformational change and is said to become activated