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Absolute lipid quantification assays are also used to determine intracellular lipid levels, in which various multi-step protocols are used to extract and quantify the total lipid content, either with high-performance liquid chromatography (HPLC) and liquid chromatography-mass spectrometry (LC-MS) methods or fluorimetric-based and colorimetric-based assays [297,298,299,300]

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Biol Cell