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Future studies should employ cell-specific knockout mouse models and tunable hydrogel-based 3D culture systems (allowing precise manipulation of ECM stiffness and ligand density) to directly test whether specific ECM alterations are sufficient to drive reproductive cell senescence and to elucidate the downstream signaling pathways involved 141,191

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The applicant calculated a final inflated average case-weighted standardized charge per case of $107,201, which exceeded the average case-weighted threshold amount of $76,389