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Brazilian Journal of Microbiology, 47, 658–664

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2.3 Antibiotic persistence assay Pneumococcal strains were cultured in BHI until mid-log phase (OD 600nm ), exposed to 20 mM H 2 O 2 for 30 min, followed by treatment with 6 mg/mL levofloxacin for 5 hours at 37°C