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In a 16-week study of nightly GHRH-(1-29) dosing in older adults, nocturnal growth-hormone output stayed elevated across the full treatment period and IGF-1 rose and remained above baseline, with no sign of the pituitary becoming desensitized to nightly stimulation over those 16 weeks (Khorram et al., 1997)

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GHRH increases intracellular cAMP that produces an increase in the synthesis of CREB proteins that bind to the two CREs (proximal and distal) of the GH promoter (Figure 7) increasing the transcription of the gene