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Mice lacking the Gpx4 allele in IECs show signs of epithelial LPO and focal neutrophilic enteritis with a granuloma-like accumulation of inflammatory cells when fed a PUFA-rich diet (65)

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The cells resuspended in binding buffer (50 mM sodium phosphate, 300 mM NaCl and 20 mM imidazole, pH 7.4) were sonicated for 10 min with middle pulse extension on ice