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Loss of methylation of the ICR1 on the paternal allele results in the phenotype of Silver Russell syndrome (SRS) which may also arise from other genetic aberrations that have not yet been linked to the IGF-II production or signaling cascade including maternal uniparental dyssomnia of chromosome 7

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This suggest the cells maybe be slightly stressed and/or some cells may have died due to removal of IL-3 in the media which is required to promote cell growth and differentiation but can influence cytokine secretion