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The third preprocessing step, data normalization, was necessary because external elements not of biological interest can impact a sample’s expression during sample preparation for sequencing

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These data are obtained by plotting the absorbance values at 500 nm during titration as a function of the logarithm of the added biotin or BioGHK concentration (Figure 4b) and interpolating the data following Equation (1): where the minimum ( F 0 ) and maximum ( F max ) absorbance values have been normalized and expressed in terms of relative percentage with respect to the maximum absorbance value recorded